Vacuolar Phosphate Transporter1 (VPT1) may transport sugar in response to soluble sugar status of grape fruits

Abstract Vacuolar Phosphate Transporter1 (VPT1)-mediated phosphate uptake in the vacuoles is essential to plant development and fruit ripening. Interestingly, here we find that VPT1 may transport sugar response soluble status of fruits. The VvVPT1 protein isolated from grape (Vitis vinifera) berries was tonoplast-localized contains SPX (Syg1/Pho81/XPR1) MFS (major facilitator superfamily) domains. Its mRNA expression significantly increased during ripening induced by sucrose. Functional analyses based on transient transgenic systems berry showed positively regulated affected hexose contents, firmness, ripening-related gene expression. proteins (Grape VvVPT1, strawberry FaVPT1, Arabidopsis AtVPT1) all low affinity for verified yeast system, while they appear different capacity, consistent with status. Thus, our findings reveal a role ripening, associated its domains direct available into vacuole, open potential avenues genetic improvement fleshy fruit.