Vaccine Design, Adaptation, and Cloning Design for Multiple Epitope-Based Vaccine Derived From SARS-CoV-2 Surface Glycoprotein (S), Membrane Protein (M) and Envelope Protein (E): In silico approach

Introduction: The SARS Coronavirus-2 (SARS-CoV-2) pandemic has become a global epidemic that increased the scientific community's concern about developing and finding counteraction against this lethal virus. So far, hundreds of thousands people have been infected by due to contamination spread. This research was therefore carried out develop potential epitope-based vaccines SARS-CoV-2 virus using reverse vaccinology immunoinformatics approaches.Material Methods: material SARS-COV2 Surface Glycoprotein (S), Membrane Protein (M), Envelope (E) were downloaded from NCBI protein database. Each undergone epitopes prediction for MHC class I epitopes, II Antibody B-cell epitopes. Selected according their antigenicity score tested allergenicity toxicity. Finally, filtered used in vaccine construction. Vaccines constructed, docked Toll-like receptor 3, Molecular Dynamic simulation. with best scores, subjected immune stimulation cloning design.Results: Three COVac-1, COVac-2, COVac-3. submitted into deep investigation. molecular dynamic simulation determines stability physical movement atoms molecules. After dynamics simulation, COVac-1 having scores. then analysis insure innate adaptive immunity. passing integrated E.coli pET-30b plasmid silico design.Conclusion:Viral pandemics are threatened face humanity today. scenario fight any is utilizing full power computational biology, especially immune-informatics, design discover new or molecules may stimulate system invader pathogens inhibit pathogen life cycle.