Useful Buffer and Gel Systems for Polyacrylamide Gel Electrophoresis
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چکیده
منابع مشابه
Useful buffer and gel systems for polyacrylamide gel electrophoresis.
Seven buffer and gel systems for polyacrylamide gel electrophoresis using discontinuous voltage and pH gradients (disc electrophoresis according to ORNSTEIN-UAVIS) and five systems using discontinuous voltage gradients at constant pH (according to ALLEN) have been compiled and characterized. Usefulness and applicability of the systems for clinical investigations are discussed and illustrated by...
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Polyacrylamide gels are typically formed by polymerization of the monomer acrylamide crosslinked to the co-monomer, N,N’-methylenebis-acrylamide, commonly called BIS. This process is a freeradical polymerization that requires an initiator, usually ammonium persulfate, and a catalyst like N,N,N’,N’-tetraethylmethylenediamine (TEMED). A distinct advantage of polyacrylamide gel systems is that by ...
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ژورنال
عنوان ژورنال: Clinical Chemistry and Laboratory Medicine
سال: 1972
ISSN: 1434-6621,1437-4331
DOI: 10.1515/cclm.1972.10.5.220