USED NASTED-PCR DETECTION OF PHYTOPLASMA CAUSING BIG BUD DISEASE ON TOMATO IN IRAQ

tomato big bud disease in lorestan province

phytoplasmal big bud is one of the important diseases of tomato (lycopersicon esculentum) in the world. the disease has been reported from iranian provinces of bushehr, isfahan, fars, khorasane razavi and ardabil. during a summer surrvey in 2010, symptoms of big bud disease was observed in tomato fields of dorood area in lorestan province. characteristic symptoms of the disease were small thick...

characterization of phytoplasmas associated with tomato big bud disease, using single-tube nested pcr

tomato is an important vegetable crop in iran. recently symptoms associated with phytoplasma disease were observed in tomato cultivars in karaj vicinity. the phytoplasmas were detected and characterized using single-tube nested pcr and rflp. the universal primer pairs amplified the target dna with expected sizes from symptomatic samples in direct pcr and single-tube nested pcr. the patterns of ...

PCR-based assay for the detection of Xanthomonas euvesicatoria causing pepper and tomato bacterial spot.

AIMS To develop a PCR-based assay for Xanthomonas euvesicatoria detection in culture and in planta. METHODS AND RESULTS A fragment of 1600 bp specific for X. euvesicatoria was found by repetitive extragenic palindromic sequence-PCR. Among the primers designed on the basis of the partially sequenced fragment, the primers Xeu2.4 and Xeu2.5 direct amplification of the expected product (208 bp) f...

Detection and identification of a phytoplasma from lucerne with Australian lucerne yellows disease

Foliar and root symptoms are described for Australian lucerne yellows (ALuY), a disease common in Australian lucerne seed crops. A phytoplasma was detected in plants exhibiting symptoms, but not in symptomless lucerne plants. Oligonucleotide primers specific to the phytoplasma 16S-23S rRNA intergenic spacer region (SR) were used in polymerase chain reaction (PCR) assays on DNA extracted from lu...

3. in Situ Detection of Sandal Spike Phytoplasma 3. in Situ Detection of Sandal Spike Phytoplasma