Use of the Coccidioides posadasii Δchs5 Strain for Quality Control in the ACCUPROBE Culture Identification Test for Coccidioides immitis
نویسندگان
چکیده
منابع مشابه
Use of the Coccidioides posadasii Deltachs5 strain for quality control in the ACCUPROBE culture identification test for Coccidioides immitis.
Coccidioides posadasii Deltachs5 is a strain that is excluded from the select agent list. Sixteen assays using test reagents from three different ACCUPROBE Coccidioides immitis culture identification test lots had an average of 132,998 relative light units (RLU), which is well beyond the 50,000-RLU positive cutoff value for the test. Coccidioides posadasii Deltachs5 is a satisfactory quality co...
متن کاملLimitation of the AccuProbe Coccidioides immitis culture identification test: false-negative results with formaldehyde-killed cultures.
The AccuProbe Coccidioides immitis culture identification test (CI test) yielded false-negative results with formaldehyde-killed C. immitis submitted to a reference laboratory. Further evaluation with pure or mixed cultures or stored, heat-killed cultures revealed the CI test to be highly sensitive and specific for C. immitis except when the cultures were pretreated with formaldehyde.
متن کاملTaxonomic and diagnostic markers for identification of Coccidioides immitis and Coccidioides posadasii.
The ribosomal Internal Transcribed Spacer (ITS) regions of the two recognized species of Coccidioides were studied using a reference set of strains that had been previously identified with species defining microsatellite polymorphisms. Unambiguous identification of the two species proved to be possible by amplifying and sequencing the ITS region. PCR-reactions are sensitive to amplification con...
متن کاملGenetic analysis of the endemic fungal pathogens Coccidioides posadasii and Coccidioides immitis in Mexico.
Coccidioidomycosis (CM) is a mycotic disease that affects mammals, including humans. Official data relative to CM in Mexico has not been collected since 1995, thus its prevalence remains unknown. The objectives of this study were to identify the predominant Coccidioides species in Mexico, infer their current geographical distribution and explore the correlation between species and clinical pres...
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انتخاب نامساعد یکی از مشکلات اساسی در صنعت بیمه است. که ابتدا در سال 1960، توسط روتشیلد واستیگلیتز مورد بحث ومطالعه قرار گرفت ازآن موقع تاکنون بسیاری از پژوهشگران مدل های مختلفی را برای تجزیه و تحلیل تقاضا برای صنعت بیمه عمر که تماما ناشی از عدم قطعیت در این صنعت میباشد انجام داده اند .وهدف از آن پیدا کردن شرایطی است که تحت آن شرایط انتخاب یا کنار گذاشتن یک بیمه گزار به نفع و یا زیان شرکت بیمه ...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2006
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.01265-06