Use of Sample Hematocrit Value to Correct Blood Tacrolimus Concentration Derived by Microparticle Enzyme Immunoassay
نویسندگان
چکیده
منابع مشابه
False positive blood tacrolimus concentration in microparticle enzyme immunoassay.
The difference in the blood concentration of tacrolimus between the assay methods, microparticle enzyme immunoassay (MEIA) and enzyme linked immunosorbent assay (ELISA) was observed in a liver transplant recipient with anemia. MEIA provided significantly higher concentration than those of ELISA (7.8+/-1.9 vs. 5.0 +/- 1.8nglml, p<0.05) while the patient had low haematocrit <25%. The difference, ...
متن کاملHPLC-microparticle enzyme immunoassay specific for tacrolimus in whole blood of hepatic and renal transplant patients.
Tacrolimus is a relatively new immunosuppressant used in organ transplantation to prevent graft rejection. However, its use is not devoid of side effects, making it important to maintain blood concentrations within therapeutic ranges. Several analytical methods are currently available for routine drug monitoring. However, these methods are based on use of the same monoclonal antibody, which als...
متن کاملLow hematocrit and serum albumin concentrations underlie the overestimation of tacrolimus concentrations by microparticle enzyme immunoassay versus liquid chromatography-tandem mass spectrometry.
BACKGROUND Rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) methods are used increasingly for tacrolimus (TRL) monitoring but show a negative difference with respect to a microparticle immunoassay (MEIA). This report examines possible reasons for this difference between methods. METHODS We collected 1156 blood samples from 277 adult and 121 pediatric recipients of liver, renal,...
متن کاملEvaluation of the tacrolimus II microparticle enzyme immunoassay (MEIA II) in liver and renal transplant recipients.
We evaluated the MEIA II with blood samples with added tacrolimus (3.0, 5.0, 11.0, and 22.0 microg/L). The assay had acceptable recoveries (99-103%) and intraday imprecision (<16.0%) across the range of concentrations studied, except for the recoveries at 3.0 microg/L (86.3%) and 5.0 microg/L (80.7%). Comparison of liver (n = 116) and renal (n = 113) patient samples measured by MEIA II against ...
متن کاملCYP3A5 genotype does not influence the blood concentration of tacrolimus measured with the Abbott immunoassay.
To the Editor: Therapeutic drug monitoring of immunosuppressants is well established as an aid to optimizing patient management after allograft transplantation. Most laboratories measure cyclosporin and tacrolimus by immunoassays. However, measured values may be affected by the presence of circulating metabolites because of the limited specificity of the antibodies used in these assays. Cyclosp...
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ژورنال
عنوان ژورنال: Biological and Pharmaceutical Bulletin
سال: 2008
ISSN: 0918-6158,1347-5215
DOI: 10.1248/bpb.31.1250