Use of Penicillium chrysogenum Mycelium as Animal Food

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Production of penicillin with waste mycelium Penicillium chrysogenum as the sole source of nitrogen.

In a preliminary communication (Ghosh, 1959) it was reported that fresh and wet mycelium of Penicillium chrysogenum, as obtained from a rotary filter in a penicillin plant, could be well utilized as the sole source of nitrogen for penicillin production by the mold either in shaken flask or in aerated and agitated large fermentors. The waste mycelium did not need any pretreatment whatsoever and ...

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The effect of nutrients on formation of penicillin by washed mycelium of Penicillium chrysogenum.

Many different substances are known to affect the yields of penicillin from growing cultures of Penicillium chrysogenum. Under the usual conditions, it is difficult to distinguish between the effect of a substance on growth of the mould (and hence the indirect effect on production of penicillin) and its direct effect on antibiotic synthesis. The use of washed mycelium (Halliday & Arnstein, 1956...

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Penicillin acyltransferase in Penicillium chrysogenum.

Isotopic exchange of (35)S between penicillins and 6-amino-penicillanic acid (6-APA) was observed in cell-free extracts of Penicillium chrysogenum. Sulfhydryl-containing compounds were required for activity. Dithiothreitol, dithioerythritol, mercaptoethanol, and glutathione served as activators. The acyltransferase was purified threefold by adsorption on calcium phosphate gel at pH 6 and elutio...

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The lactic dehydrogenase of Penicillium chrysogenum.

A soluble, cytochrome linked flavoprotein which oxidizes lactic acid to pyruvic acid has been isolated from Penicillium chrysogenum, strain NRRL 1951 -B25. The reduction of cytochrome c by a flavin enzyme has precedence in the work of Horecker and Heppel (1949) and Mlorell (1952) who have shown that xanthine oxidase can reduce cytochrome c. Morell reported the enzyme to be inhibited strongly by...

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Ballistic disruption of Penicillium chrysogenum cells.

The use of glass beads in a high-speed mixing device to rupture organisms was applied to molds. The use of a mixer in which the propellor shaft enters from the top into a metal mixing chamber made it possible to immerse the whole device in a salt water and ice mixture so that the temperature of the glass-bead slurry could be kept below 5 C without difficulty. Mycelia, glass beads, and buffer in...

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ژورنال

عنوان ژورنال: Applied Microbiology

سال: 1965

ISSN: 0003-6919

DOI: 10.1128/aem.13.2.262-266.1965