Urea biosensor based on immobilization of urease on ZnO nanoparticles
نویسندگان
چکیده
منابع مشابه
A Conductometric Urea Biosensor by Direct Immobilization of Urease on Pt Electrode
Urease was immobilized on platinum electrode both by chemical binding and electropolymerization.The conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 M and linear dynamic range from 4.9×10-5 to 5.8×10-3 M for urea concentration when the enzyme is covalently immobilized on Pt electrodes. Conductometric transducers respo...
متن کاملa conductometric urea biosensor by direct immobilization of urease on pt electrode
urease was immobilized on platinum electrode both by chemical binding and electropolymerization.the conductometric urea biosensor thus prepared showed a detection limit of 4.9×10-5 m and linear dynamic range from 4.9×10-5 to 5.8×10-3 m for urea concentration when the enzyme is covalently immobilized on pt electrodes. conductometric transducers respond to the changes in ionic strength thereby le...
متن کاملCovalent immobilization of urease on polypyrrole microspheres for application as a urea biosensor
Urease has been covalently immobilized on polypyrrole microspheres chemically linked to conducting polypyrrole-polyvinyl sulfonate (PPY-PVS) films. These films were electrochemically prepared during 5 7 min at a constant current of 2 mA using indium tin oxide (ITO) glass plates as the working electrode, and a standard calomel electrode as the reference electrode. Urease covalently linked to pol...
متن کاملSelective determination of urea using urease immobilized on ZnO nanowires
Well-aligned zinc oxide (ZnO) nanowire arrays were fabricated on gold-coated plastic substrates using a low-temperature aqueous chemical growth (ACG) method. The ZnO nanowire arrays with 50-130 nm diameters and ~1 μm in lengths were used in an enzymebased urea sensor through immobilization of the enzyme urease that was found to be sensitive to urea concentrations from 0.1 mM to 100 mM. Two line...
متن کاملImpedimetric Urea Biosensor Based on Modified Gold Electrode with Urease Immobilized on Glutathione Layer
In this work, a glutathione (GSH) modified gold microelectrode was used for the covalent immobilization of urease biomolecules via the glutaraldehyde-coupling agent. The self-assembled monolayers (SAMs) onto the gold surface was investigated by using the electrochemical impedance spectroscopy measurements (EIS). Before urease grafting, a significant interaction was noticed between urea and the ...
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ژورنال
عنوان ژورنال: Oriental Journal of Chemistry
سال: 2015
ISSN: 0970-020X,2231-5039
DOI: 10.13005/ojc/310284