Urea and Guanidine Hydrochloride Denaturation of Ribonuclease, Lysozyme, α-Chymotrypsin, and b-Lactoglobulin
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منابع مشابه
Urea and Guanidine Hydrochloride Denaturation of Ribonuclease, Lysozyme, &Zhymotrypsin, and @Lactoglobulin*
The unfolding of ribonuclease, lysozyme, a-chymotrypsin, and goat P-lactoglobulin by urea and guanidine hydrochloride (GmHCl) has been followed with the use of optical rotation measurements. Urea denaturation leads to a more negative rotation for each protein than does GmHCl denaturation, but the concentration dependence is such that the rotations are almost identical in the absence of denatura...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1974
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(20)79739-5