Unveiling Chloroplast RNA Editing Events Using Next Generation Small RNA Sequencing Data

Unveiling Chloroplast RNA Editing Events Using Next Generation Small RNA Sequencing Data

Organellar RNA editing involves the modification of nucleotide sequences to maintain conserved protein functions, mainly by reverting non-neutral codon mutations. The loss of plastid editing events, resulting from mutations in RNA editing factors or through stress interference, leads to developmental, physiological and photosynthetic alterations. Recently, next generation sequencing technology ...

RNA Sequencing with Next-Generation Sequencing

Sequencing of RNA has been an important application of DNA sequencing technology since its invention. RNA is usually sequenced by first converting it to complementary DNA (cDNA) with the reverse transcriptase enzyme (RNA-dependent DNA polymerase). Reverse transcriptase was originally isolated from Rous sarcoma retrovirus and Rauscher mouse leukemia retrovirus (R-MLV) by Baltimore (1970) and ind...

Assessing the 5S ribosomal RNA heterogeneity in Arabidopsis thaliana using short RNA next generation sequencing data.

In eukaryotes, ribosomal 5S rRNAs are products of multigene families organized within clusters of tandemly repeated units. Accumulation of genomic data obtained from a variety of organisms demonstrated that the potential 5S rRNA coding sequences show a large number of variants, often incompatible with folding into a correct secondary structure. Here, we present results of an analysis of a large...

RNA Isolation (Small RNA Extraction). Fatemeh Mehrpooyan*

Characterizing the small RNA transcriptome associated with COPD and ILD using next-generation sequencing

Background Despite the increasing public health burden associated with chronic obstructive pulmonary disease (COPD) and interstitial lung disease (ILD), the molecular mechanisms responsible for the pathogenesis of these diseases remain unclear. The goal of this study was to comprehensively profile the lung small RNA transcriptome via next-generation sequencing, and elucidate microRNAs that migh...