منابع مشابه
Universal linker phosphoramidite
Universal Linker phosphoramidite was synthesized and employed for conversion of conventional nucleoside bound solid phase into the universal support. The latter was successfully tested in preparation of the 20mer oligonucleotide.
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The application of a universal allyl linker, 9-O-(4,4'-dimethoxytrityl)-10-undecenoic acid, to the solid phase synthesis of RNA molecules is described. Use of this linker simplifies significantly the isolation and purification steps in RNA synthesis. The linker is universal in that it does not contain a nucleoside. The 3'terminal nucleoside is instead attached to the support in the first coupli...
متن کاملA new universal linker for solid phase DNA synthesis.
A method is described as an alternative to the use of nucleoside pre-functionalized supports for DNA synthesis. The procedure should allow the generation of 3'-OH terminal moieties of any natural or modified DNA fragment using a single derivatized solid support material. The method utilizes 1-O-(4,4' dimethoxytrityl)-2- O-succinoyl-3-N-allyloxycarbonylpropane immobilized on amino-propyl CPG fol...
متن کاملAsymmetric hydrogenation using monodentate phosphoramidite ligands.
Monodentate phosphoramidites are excellent ligands for Rh-catalyzed asymmetric hydrogenations of substituted olefins. Enantioselectivities between 95 and 99% were obtained in the asymmetric hydrogenation of protected alpha- and beta-dehydroamino acids and esters, itaconic acid and esters, aromatic enamides, aromatic enol esters, aromatic and aliphatic enol carbamates, and alpha-substituted cinn...
متن کاملSelective O-phosphitilation with nucleoside phosphoramidite reagents.
In contrast to tetrazole, pyridine hydrochloride/imidazole converts nucleoside phosphoramidites to intermediates that show a high preference for phosphitilating hydroxyl groups relative to nucleoside amino groups. Use of this activating agent and incorporation of a pyridine hydrochloride/aniline wash step in the synthetic cycles permit synthesis of mixed base twenty-mer oligonucleotides from nu...
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ژورنال
عنوان ژورنال: Arkivoc
سال: 2008
ISSN: 1551-7012
DOI: 10.3998/ark.5550190.0010.315