Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
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چکیده
منابع مشابه
Universal Human Papillomavirus Typing Assay: Whole-Genome Sequencing following Target Enrichment
We designed a universal human papillomavirus (HPV) typing assay based on target enrichment and whole-genome sequencing (eWGS). The RNA bait included 23,941 probes targeting 191 HPV types and 12 probes targeting beta-globin as a control. We used the Agilent SureSelect XT2 protocol for library preparation, Illumina HiSeq 2500 for sequencing, and CLC Genomics Workbench for sequence analysis. Mappi...
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Human papillomavirus (HPV) is a strongly conserved DNA virus, high-risk types of which can cause cervical cancer in persistent infections. The most common type found in HPV-attributable cancer is HPV16, which can be subdivided into four lineages (A to D) with different carcinogenic properties. Studies have shown HPV16 sequence diversity in different geographical areas, but only limited informat...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 2017
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.02132-16