Unearthing Hidden Genomic Data in Solid Tumor Samples: Are Your FFPE Samples Revealing All?
نویسندگان
چکیده
منابع مشابه
Simultaneous detection of BRCA mutations and large genomic rearrangements in germline DNA and FFPE tumor samples
The development of breast and ovarian cancer is strongly connected to the inactivation of the BRCA1 and BRCA2 genes by different germline and somatic alterations, and their diagnosis has great significance in targeted tumor therapy, since recently approved PARP inhibitors show high efficiency in the treatment of BRCA-deficient tumors. This raises the need for new diagnostic methods that are cap...
متن کاملDeterminants of RNA Quality from FFPE Samples
The large archives of formalin-fixed paraffin-embedded (FFPE) tissue specimens that exist are a highly valuable source of sample material for molecular biological analysis, including gene expression profiling. However, current data on adverse effects of standard pathological practice on the usefulness of biomolecular analytes obtained from such archived specimens is largely anecdotal. Here, we ...
متن کاملTechnical Note: RNA Extraction from FFPE Samples
Existing methods rely primarily on heat to remove crosslinks and adducts, which is only partially effective and leads to additional fragmentation of labile nucleic acids such as RNA. In contrast, the catalytic CAT5TM technology developed by Cell Data Sciences and included in the RNAstormTM kit greatly accelerates the removal of formaldehyde damage and allows the use of milder conditions, result...
متن کاملmiRNA Extraction and Profiling on FFPE Samples
Introduction RNA silencing is a mechanism of gene regulation whereby small double-stranded RNAs (dsRNAs) of 21–18 nucleotides in length cause mRNA degradation, mRNA translation, or chromatin modification [1]. In the past years, more than 500 novel genes have been identified in animals and plants encoding small transcripts that can form small dsRNA hairpin structures [2–4]. From these hairpin pr...
متن کاملAre samples in your freezer still good for biomarker discovery?
Disease markers play an important role in patient care.1,2 Diagnostic markers can be used for detecting diseases, predicting response to a therapy, making prognosis for a patient, or estimating the outcome of a treatment. Despite great progress in instrumentation and analytical techniques, few new biomarkers have been discovered during the past decade. Some reasons for slow progress are the low...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Science
سال: 2013
ISSN: 0036-8075,1095-9203
DOI: 10.1126/science.341.6151.1257-c