Uncovering biologically significant lipid isomers with liquid chromatography, ion mobility spectrometry and mass spectrometry

Evaluation of capillary liquid chromatography-electrospray ionization ion mobility spectrometry with mass spectrometry detection.

Due to the proteomics revolution, multi-dimensional separation and detection instruments are required to evaluate many peptides and proteins in single samples. In this study, electrospray ionization (ESI) ion mobility spectrometry (IMS) was evaluated as an additional separation after HPLC separations. Common HPLC mobile phase compositions (solvents, acid modifiers, and buffers) were assessed fo...

Ion Mobility Mass Spectrometry

for more complex analysers for automated sample processing and plant control. The separation time will continue to decrease; in the past there has been limited interest in fast separations but this could change as automated sample processing is developed. Increasing use of coupled techniques such as GC-GC, liquid chromatography-GC, and supercritical Suid chromatography-GC for the separation of ...

Liquid Chromatography - Mass Spectrometry

In this article, selected examples of applications of liquid chromatography coupled to mass spectrometry are given. The examples include the analysis of i) impurities in manufactured, pharmaceutical or synthesis products, ii) polyphenols in natural products, and iii) phytohormones in plant extracts. Finally, examples of applications of molecular characterization via flow injection analysis by e...

Ion Mobility-Mass Spectrometry

Liquid phase ion mobility spectrometry.

A novel analytical method, called Liquid Phase Ion Mobility Spectrometry (LiPIMS) was demonstrated, where aqueous phase analytes were ionized and introduced into non-aqueous liquids, transported by an external electric field from the point of generation to a collection electrode. Ions were produced from a unique liquid phase ionization process, called Electrodispersion Ionization. Spectra of an...