ULTRAVIOLET MICROSCOPY OF PANCREATIC ACINAR NUCLEI FIXED IN POTASSIUM PERMANGANATE
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چکیده
منابع مشابه
Ultraviolet Microscopy of Pancreatic Acinar Nuclei Fixed in Potassium Permanganate
The fixation of rat pancreas in potassium permanganate according to Luft (1) imparts a brown coloration and a strong ultraviolet absorption to the cytoplasm of the exocrine cells, but only a weak one to the nuclei. Within the latter an even paler zone surrounding the nucleoli can be observed. After the removal of the stain by means of oxalic acid, ultraviolet microscopy and the Feulgen technic ...
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The action of buffered potassium permanganate as a fixative for electron microscopy has been investigated. Electron contrast has been shown to be produced by the deposition of granular material in the tissue. The particles are about 50 A in diameter. Actual fixation of the tissue is performed by the dehydrating alcohol. Histochemical studies have shown that RNA and histones are removed, whereas...
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The control of regulated exocytosis is still largely unknown in epithelial cells (12). While much progress has been made in understanding constitutive secretion we still know only in outline the mechanisms of secretory granule fusion (5). Progress requires methods of real-time imaging of granule exocytosis and in recent years a variety of optical methods have been applied towards understanding ...
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ژورنال
عنوان ژورنال: The Journal of Biophysical and Biochemical Cytology
سال: 1961
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb.9.3.716