Ultraviolet irradiation accelerates apoptosis in human polymorphonuclear leukocytes: protection by LPS and GM-CSF

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Effects of GM-CSF, IL-3, and GM-CSF/IL-3 fusion protein on apoptosis of human myeloid leukemic cell line Tf-1 induced by irradiation.

AIM To observe the effects of three cytokines on the apoptosis of Tf-1 cells induced by gamma irradiation and investigate the relationship between apoptosis and caspase-3 activity. METHODS Different cytokines GM-CSF, IL-3 and GM-CS/IL-3 fusion protein were added into the irradiated Tf-1 cells. MTT assay, morphology, flow cytometry, and DNA fragmentation assay were used to observe the effects ...

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ژورنال

عنوان ژورنال: Journal of Leukocyte Biology

سال: 1997

ISSN: 0741-5400

DOI: 10.1002/jlb.62.4.517