Ultrastructure and Development of Vitrified/Warmed Bovine Oocytes Matured with 9-cis Retinoic Acid
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چکیده
منابع مشابه
Ultrastructure and Development of Vitrified/Warmed Bovine Oocytes Matured with 9-cis Retinoic Acid
In this work we analyze the effects of vitrification on the ultrastructure and developmental ability of bovine oocytes matured in the presence of 9-cis-retinoic acid (RA). Bovine cumulus oocyte complexes (COCs) were matured in a basic medium containing 10% fetal calf serum (FCS), 9-cis-RA or polyvinyl alcohol (PVA). Mature oocytes were vitrified using the Open Pulled Straw method with minor mod...
متن کاملPregnancies and improved early embryonic development with bovine oocytes matured in vitro with 9-cis-retinoic acid.
Retinoids have an important role in cell growth, morphogenesis and differentiation. In the present study the developmental potential of bovine oocytes was examined after in vitro maturation in the presence of 9-cis-retinoic acid, a vitamin A metabolite, at 5 nmol l(-1) in chemically defined conditions. Experiments studied early in vitro development, blastocyst differential cell counts and the c...
متن کاملUltrastructure of in vitro Matured Human Oocytes
BACKGROUND Approximately 20% of recovered oocytes are immature and discarded in intracytoplasmic sperm injection (ICSI) procedures. These oocytes represent a potential resource for both clinical and basic science application. OBJECTIVE The aim of this study was to evaluate the ultrastructure architecture of in vitro matured human oocytes using transmission electron microscopy (TEM). MATERIA...
متن کامل9-cis Retinoic acid inhibits cumulus cell apoptosis during the maturation of bovine cumulus-oocyte-complexes.
Cumulus cell (CC) apoptosis is inversely correlated with embryonic development in vitro. Therefore, inhibition of CC apoptosis is important for proper embryonic development and quality. Retinoic acids (all-transRA and 9-cisRA) are natural components of retinoids, and 9-cisRA is the physiologically active metabolite of retinoic acid in vitro. During in vitro maturation, 9-cisRA enhances oocyte c...
متن کاملEffects of Novel Retinoic Acid Compound, 9-cis-Retinoic Acid, on Proliferation, Differentiation, and Expression of Retinoic Acid Receptor-a
Retinoic acid modulates proliferation and differentiation of a wide variety of normal and leukemic cells through two distinct families of transcriptional factors: the retinoic acid receptors (RARs) and the retinoid X receptors (RXRs). A stereoisomer of retinoic acid, 9-cis-retinoic acid, is a highaffinity ligand for RXR and binds efficiently to RAR. In contrast, all-trans-retinoic acid interact...
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ژورنال
عنوان ژورنال: Cell Preservation Technology
سال: 2006
ISSN: 1538-344X,1557-8119
DOI: 10.1089/cpt.2006.4.123