منابع مشابه
Reversible polymerization and ultracentrifugal purification of actin.
Actin is the second major structure protein of muscle. It is characterized by its ability to combine with myosin and to occur in two forms, globular and fibrous actin (9, 10, 3), which are indicated as Gand Factin. The transition of Ginto F-actin is brought about by addition of salt at neutral or weakly alkaline reaction (9, 10, 4). The present paper describes a method of purifying actin by pre...
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By ultracentrifugation of 30 ml of highly dilute suspensions of herpes simplex virus (HSV) directly onto monolayer cultures grown in centrifuge tubes, infectivity was significantly greater than without centrifugation. Ultracentrifugation at 20,000 to 25,000 rpm (28,000 to 45,000 X g) for 1.5 to 2.3 h was utilized with good preservation of cultures. With low-speed centrifugation at 3,000 rpm (1,...
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Ultracentrifugal examination of staphylococcal alpha toxin at different stages of purification showed the presence of a major component having a sedimentation coefficient of 2.8S, present to the extent of more than 90% of the sample, and identifiable with active toxin. Several minor components having S(20,w) values of 11.5S, 8.5S, and 2.0S were detected. The 11.5S component presumably is identi...
متن کاملUltracentrifugal Evidence for a Ring Structure
By velocity sedimentation, in appropriate solvents, the presence of two discrete components can be demonstrated in preparations of the DNA of bacteriophage <jJX174. The major, faster-moving 81 and the slower 82 are present under conditions which exclude the possibility of hydrogen-bond formation. It can be shown, either by treatment with pancreatic deoxyribonuclease or by thermal inactivation, ...
متن کاملUltracentrifugal studies of lipoproteins of human serum.
In spite of several extensive ultracentrifugal studies of human sera by Mutaenbecher, McFarlane, and Pedersen, the interpretation of the patterns observed has remained in doubt. Specifically, the greatest difficulties encountered by previous workers have arisen in their efforts to study undiluted human sera. McFarlane (1) referred to marked distortions in the pattern observed with undiluted hum...
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ژورنال
عنوان ژورنال: Nature
سال: 1973
ISSN: 0028-0836,1476-4687
DOI: 10.1038/245276b0