Ultra-structural analysis and morphological changes during the differentiation of trophozoite to cyst in Entamoeba invadens

Entamoeba histolytica, a pathogenic parasite, is the causative organism of amoebiasis and uses human colon to complete its life cycle. It destroys intestinal tissue leading invasive disease. Since it does not form cyst in culture medium, reptilian parasite invadens serves as model system study encystation. Detailed investigation on mechanism formation, information ultra-structural changes wall formation during encystation are still lacking E. invadens. Here, we used electron microscopy ultrastructural showed that increase heterochromatin patches deformation nuclear shape were early events These peaked at ∼20 h post induction, normal morphology was restored by 72 h. Two types cellular structures visible 16 One densely stained consisted cytoplasmic mass with clearly nucleus. The other membranous shells large vacuoles scant cytoplasm. former structure developed into mature while latter lost after 20 h, This opens up possibilities for further mechanisms involved this novel process.