Ultra-microdetermination of amino acids by microbioassay, applying lactic acid assay with lactate dehydrogenase.
نویسندگان
چکیده
منابع مشابه
Amino acid sequence of dogfish muscle lactate dehydrogenase.
Lactate dehydrogenase isolated from vertebrate tissues catalyzes the reversible oxidation of L-lactic acid to pyruvic acid. The catalysis requires nicotinamide adenine dinucleotide (NAD’) as a coenzyme and is specific for the A-hydrogen of the nicotinamide ring (1) (Fig. 1). Although the enzyme is tetrameric, no cooperative effects have been observed between subunits (2). Each identical subunit...
متن کاملInterference by branched-chain amino acids in the assay of alanine with alanine dehydrogenase.
Commercial preparations of alanine dehydrogenase from Bacillus subtilis are contaminated to varying extents with activity towards branched-chain amino acids. The Km values for these amino acids are of the same order as for L-alanine (about 10(-3)M). The branched-chain amino acid dehydrogenase activity is lost on dialysis for 2--4h against water or 2mM-EDTA.
متن کاملAntagonisms in the utilization of d-amino acids by lactic acid bacteria. IV. d-Aspartic acid.
Investigations with glutamic acid, methionine, and alanine have shown that n-amino acid utilization by lactic acid bacteria may be inhibited by specific antimetabolites (l-4). This has been taken to indicate that a conversion of o-amino acid substrate to the L form precedes its utilization, and that this process may be blocked by an appropriate inhibitor. Both Land n-alanines have been shown to...
متن کاملThe tentative amino acid sequencing of lactate dehydrogenase C4 by X-ray diffraction analysis.
A tentative amino acid sequence of mouse testicular lactate dehydrogenase C4 was deduced from an electron density map and comparison with five other known lactate dehydrogenase sequences. The amino acid composition determined by chemical analysis agrees reasonably well with the present results. Necessary changes in amino acids were largely conservative and confined to the external portions of t...
متن کاملEfficient production of L-Lactic acid by metabolically engineered Saccharomyces cerevisiae with a genome-integrated L-lactate dehydrogenase gene.
We developed a metabolically engineered yeast which produces lactic acid efficiently. In this recombinant strain, the coding region for pyruvate decarboxylase 1 (PDC1) on chromosome XII is substituted for that of the l-lactate dehydrogenase gene (LDH) through homologous recombination. The expression of mRNA for the genome-integrated LDH is regulated under the control of the native PDC1 promoter...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Chemical and Pharmaceutical Bulletin
سال: 1974
ISSN: 0009-2363,1347-5223
DOI: 10.1248/cpb.22.2487