Ultra-large field-of-view two-photon microscopy
نویسندگان
چکیده
منابع مشابه
Ultra-large field-of-view two-photon microscopy.
We present a two-photon microscope that images the full extent of murine cortex with an objective-limited spatial resolution across an 8 mm by 10 mm field. The lateral resolution is approximately 1 µm and the maximum scan speed is 5 mm/ms. The scan pathway employs large diameter compound lenses to minimize aberrations and performs near theoretical limits. We demonstrate the special utility of t...
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Two-Photon Selective Plane Illumination Microscopy (2p-SPIM) was recently demonstrated to be an interesting alternative to its commonly used single-photon counterpart [1]. Although this technique is ideal for accessing larger penetration depth in biological samples it is not optimal in terms of efficiency of fluorescence excitation due to the reduced intensities involved [2]. To overcome this l...
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Common microscope objectives have fields of view (FOVs) of less than a few millimeters because of limits imposed by optical aberrations. To scale up the FOVs, additional lens elements and heroic design efforts are required to compensate for the aberrations, leading to reduced transmissions and higher system costs. Such FOV limitations have thus become amajor bottleneck in microscopy for large-s...
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We have implemented continuous-wave two-photon excitation of near-UV absorbing fluorophores in a scanning near-field optical microscope (SNOM). The 647-nm emission of an Ar-Kr mixed gas laser was used to excite the UV-absorbing DNA dyes DAPI, the bisbenzimidazole Hoechst 33342, and ethidium bromide in a shared aperture SNOM with uncoated fiber tips. Polytene chromosomes of Drosophila melanogast...
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ژورنال
عنوان ژورنال: Optics Express
سال: 2015
ISSN: 1094-4087
DOI: 10.1364/oe.23.013833