UDP-glucuronosyltransferase activity and bilirubin conjugation in the bullfrog
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چکیده
منابع مشابه
Maximal biliary secretion of bilirubin in the anaesthetized rat: dependence on UDP-glucuronosyltransferase activity.
1. Hepatic bilirubin UDP-glucuronosyl transferase activity in heterozygous Gunn rats (Jj) was only two-thirds of that in the parent Wistar rats (JJ). The former excreted only 30% of their bilirubin conjugates into bile as diglucuronide in contrast to 48% in the Wistar rats. As similar changes have been observed in Gilbert's syndrome, heterozygous Gunn rats can be used as a model for this disord...
متن کاملHepatic microsomal bilirubin UDP-glucuronosyltransferase. The kinetics of bilirubin mono- and diglucuronide synthesis.
Hepatic biotransformation of bilirubin to the hydrophilic species bilirubin mono- (BMG) and diglucuronide (BDG) by microsomal bilirubin UDP-glucuronosyl-transferase (GT) is a prerequisite for its physiologic excretion into bile. The reaction mechanism of bilirubin-GT and the access of bilirubin and BMG (the intermediate substrate) to the active site of bilirubin-GT are undefined. Highly purifie...
متن کاملBilirubin glucuronidation by intact Gunn rat fibroblasts expressing bilirubin UDP-glucuronosyltransferase.
Crigler-Najjar (CN) disease is an inherited disorder of bilirubin metabolism. The disease is caused by a deficiency of the hepatic enzyme bilirubin UDP-glucuronosyltransferase (B-UGT). Patients with CN disease have high serum levels of the toxic compound, unconjugated bilirubin. The only defect in bilirubin metabolism of CN patients is the absence of B-UGT activity. The transplantation of cells...
متن کاملSex differences in UDP-glucuronosyltransferase 2B17 expression and activity.
UDP-glucuronosyltransferases (UGTs) are enzymes involved in the metabolism of steroid hormones, carcinogens, cancer chemotherapy agents, and addictive agents from cigarettes. Because the UGT2B family of genes has been linked to hormonal regulation in human cell lines in vitro, we hypothesized that there may be sex-related differences in the expression and activity of these genes in human tissue...
متن کاملAn investigation of the transverse topology of bilirubin UDP-glucuronosyltransferase in rat hepatic endoplasmic reticulum.
Bilirubin UDP-glucuronosyltransferase (UDPGT) activity in sealed hepatic microsomes from clofibrate-treated rats was highly latent and was fully expressed by disruption of vesicles with detergents. Antibodies raised against purified bilirubin UDPGT were used to study the transmembrane orientation of the protein to provide a molecular understanding of the UDPGT latency. Immunoblot analysis of se...
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ژورنال
عنوان ژورنال: Biochemical Journal
سال: 1983
ISSN: 0306-3283
DOI: 10.1042/bj2120265