Ubiquitin Degradation of the AICAR Transformylase/IMP Cyclohydrolase Ade16 Regulates the Sexual Reproduction of Cryptococcus neoformans

F-box protein is a key of the SCF E3 ubiquitin ligase complex, responsible for substrate recognition and degradation through specific interactions. Previous studies have shown that proteins play crucial roles in Cryptococcus sexual reproduction. However, molecular mechanism by which regulate reproduction C. neoformans unclear. In study, we discovered AICAR transformylase/IMP cyclohydrolase Ade16 as Fbp1. Through interaction stability experiments, demonstrated To examine role ADE16 neoformans, constructed iADE16 strains ADE16OE to analyze function Ade16. Our results revealed had smaller capsule showed growth defects under NaCl, while were sensitive SDS but not Congo red, consistent with stress phenotype fbp1Δ strains, indicating intracellular expression level after overexpression was similar FBP1 deletion. Interestingly, although can produce basidiospores normally, mating mycelia mating, fbp1Δmutant suggesting Fbp1 likely modulation A fungal nuclei development assay failed fuse bilateral plays regulation meiosis during mating. summary, our findings new determinant factor involved related post-translational cyclohydrolase.