Two-step purification of epilactose produced by cellobiose 2-epimerase from Caldicellulosiruptor saccharolyticus

Epilactose is a functional sugar that can be produced from lactose using cellobiose 2-epimerase and it considered developing prebiotic. In sense, the development of strategies to produce purify epilactose key for its wider use in food industry. The aim this work was establish food-grade purification strategy suitable scaled-up an industrial level. Firstly, by enzymatic epimerization reaction catalyzed recombinant Caldicellulosiruptor saccharolyticus Saccharomyces cerevisiae. Then, remove unreacted lactose, screening study performed find β-galactosidase enzyme with high hydrolysis capacity but low ability convert epilactose. elimination generated monosaccharides then attempted microbial treatment different microorganisms activated charcoal. baker’s yeast S. cerevisiae proven most microorganism glucose galactose removal mixture. Overall, attractive two-step process recovery established, resulting purity yield 87% 76.4%, respectively. Additionally, INFOGEST 2.0 static vitro simulation gastrointestinal digestion used, first time, assess resistance (77% resistance) upper tract conditions, reinforcing potential used as