Two-level factorial screening for influence of temperature, pH, and aeration on production of Serratia marcescens nuclease
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چکیده
منابع مشابه
study the effects of low level laser diode on no production and healing of diabetic and cortisone acetate and normal wounded sd rats
nitric oxide (no) is a small molecule synthesized by most of mammalian cells with diverse biological activities including vasodilatation, host defense and wound healing. impaired wound healing is a common occurrence among diabetics and patients receiving glucocorticoid therapy. on the other hand the application of laser in biomedical area have been increased. thus, the current studies were desi...
15 صفحه اولEffect of Ph on the Formation of Exocellular Nuclease in Aging Broth Cultures of Serratia Marcescens.
Eaves, George N. (Wayne State University College of Medicine, Detroit, Mich.) and Charles D. Jeffries. Effect of pH on the formation of exocellular nuclease in aging broth cultures of Serratia marcescens. J. Bacteriol. 85:1194-1196. 1963.-The formation of exocellular nuclease by Serratia marcescens occurred in the stationary phase of growth and was not associated with cell multiplication. An in...
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The specificity of an extracellular nuclease from Serraiia marcescens was examined with both RNA and DNA as substrates. The enzyme was found to act in a predominantly endonucleolytic manner with less than 2% of the degradation products being mononucleotides. Both DNA and RNA were degraded to di-, tri-, and tetranucleotides terminating in 5’-phosphoryl ends. The enzyme had the unusual property o...
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Differential secretion of isoforms of Serratia marcescens extracellular nuclease.
Extracellular secretion of the Serratia marcescens nuclease occurs in a two-step process: (i) rapidly to the periplasm via a signal sequence-dependent pathway and then (ii) slowly to the extracellular growth medium without cell lysis. There are two major isoforms of the nuclease in the culture supernatant of S. marcescens. We have isolated, purified, and determined the sequences of both isoform...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 1987
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.53.10.2593-2596.1987