Two-Dimensional Polyacrylamide Gel Electrophoresis of Envelope Proteins of Escherichia coli
نویسندگان
چکیده
منابع مشابه
Internal standards for molecular weight determinations of proteins by polyacrylamide gel electrophoresis. Applications to envelope proteins of Escherichia coli.
A new technique was devised to be used with molecular weight determinations of proteins on polyacrylamide gels. Internal standards consisting of proteins covalently linked to l-dimethylaminonaphthalene-S-sulfonyl(DANS-) groups were prepared. DANSderivatives of insulin and monomer, dimer, trimer, tetramer, pentamer, and hexamer of egg white lysozyme and serum albumin covered molecular weights fr...
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The recent trend in science is to assay as many biological molecules as possible within a single experiment. This trend is evident in proteomics where the aim is to characterize thousands of proteins within cells, tissues, and organisms. While advances in mass spectrometry have been critical, developments made in two-dimensional PAGE (2D-PAGE) have also played a major role in enabling proteomic...
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ژورنال
عنوان ژورنال: Applied Microbiology
سال: 1975
ISSN: 0003-6919
DOI: 10.1128/am.29.3.405-413.1975