Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis a antigen
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Two competitive enzyme immunoassays for the detection of IgG class antibodies to hepatitis A antigen.
Two competitive enzyme immunoassays (EIA) techniques were developed: in the first (COMP-1), test sera were added together with HAV antigen on anti-HAV IgG-coated wells followed by an anti-HAV HRP conjugate; in the second (COMP-2), test sera and anti-HAV HRP conjugate competed for HAV epitopes previously adsorbed to anti-HAV IgG-coated wells. Both procedures used tetramethylbenzidine (TMB) as a ...
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Departamento de Clínica Médica, Universidade Federal de Mato Grosso, Rua L s/ no, 78070-000 Cuiabá, MT, Brasil *Fundação Nacional de Saúde, Av. Getúlio Vargas 867, 78045-720 Cuiabá, MT, Brasil **Departamento de Virologia, Instituto Oswaldo Cruz, Av. Brasil 4365, 21045-900 Rio de Janeiro, RJ, Brasil ***Hospital Prof. Edgar Santos, Universidade Federal da Bahia, Rua João das Botas s/ no, 40110-16...
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Serology remains critical for diagnosing hepatitis D virus (HDV) infection, which affects 15 to 20 million people worldwide, but the literature on characterizing commercial enzyme immunoassays (EIAs) dates back to 15 years ago. We evaluated 2 commercial EIAs currently available for detecting anti-HDV antibodies. The DiaSorin assay demonstrated 100% sensitivity and specificity. Using a modified ...
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To determine suitability for national serosurveys, we compared two commercial enzyme-linked immunosorbent assays (ELISAs) for mumps antibody, Enzygnost Anti-Parotitis-Virus/IgG (which uses a whole-virus antigen) and Microimmune Mumps IgG Screen ELISA (which uses a recombinant nucleoprotein antigen), by testing 1,915 opportunistically collected sera submitted to diagnostic laboratories across Au...
متن کامل[Comparative evaluation of two enzyme immunoassays for detection of immunoglobulin G antibodies to mumps virus].
Two enzyme immunoassays (ELISA) for mumps antibody detection using the Enzygnost (Germany) and Parotit-screen (Russia) were comparatively assayed using sera of randomly assigned 70 healthy young adult volunteers. The neutralization test (NT) was performed for all sera using mumps viruses (MVs) of the relevant strains Enders and Leningrad-3. The proportion of positive results was significantly h...
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ژورنال
عنوان ژورنال: Revista da Sociedade Brasileira de Medicina Tropical
سال: 1991
ISSN: 0037-8682
DOI: 10.1590/s0037-86821991000200003