TwistAmp® Liquid: a versatile amplification method to replace PCR
نویسندگان
چکیده
منابع مشابه
Method To Detect Only Live Bacteria during PCR Amplification.
Ethidium monoazide (EMA) is a DNA cross-linking agent and eukaryotic topoisomerase II poison. We previously reported that the treatment of EMA with visible light irradiation (EMA + Light) directly cleaved chromosomal DNA of Escherichia coli (T. Soejima, K. Iida, T. Qin, H. Taniai, M. Seki, A. Takade, and S. Yoshida, Microbiol. Immunol. 51:763-775, 2007). Herein, we report that EMA + Light rando...
متن کاملSpotlight: [Pyridine-SO3H]X: As a multi-versatile ionic liquid
Sima Kalhor was born in Qom, Iran in1988. He received his B.Sc. in applied Chemistry (2012) from Islamic Azad University Qom and M.Sc. in Organic Chemistry (2014) at Islamic Azad University Qom, Iran. Also, she is currently working towards her Ph.D. student in Organic Chemistry (2016) under the supervision of Prof. Mohammad Ali Zolfigol. Her research interest is the synthesis, characterization ...
متن کاملA versatile real-time PCR method to quantify bovine contamination in buffalo products
Fraudulent species substitution in food products is a reality in many markets around the world. The consequences are serious and impact governments, consumers and producers. Molecular methods, including PCR-based techniques, are available to prevent species substitutions and to authenticate food content. Here, we describe a method for calculating the bovine and buffalo content in milkand meatde...
متن کاملImproved PCR method for amplification of GC-rich DNA sequences.
Most housekeeping genes, tumor-suppressor genes, and approx 40% of tissue-specific genes contain G+C sequences in their promoter region that were very difficult to amplify. In this report, we propose an improved polymerase chain reaction (PCR) method to be used for successful amplification of the tissue factor pathway inhibitor (TFPI)-2 gene promoter region that exhibit >70% G+C content in a se...
متن کاملOligoribonucleotide (ORN) Interference-PCR (ORNi-PCR): A Simple Method for Suppressing PCR Amplification of Specific DNA Sequences Using ORNs
Polymerase chain reaction (PCR) amplification of multiple templates using common primers is used in a wide variety of molecular biological techniques. However, abundant templates sometimes obscure the amplification of minor species containing the same primer sequences. To overcome this challenge, we used oligoribonucleotides (ORNs) to inhibit amplification of undesired template sequences withou...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Methods
سال: 2018
ISSN: 1548-7091,1548-7105
DOI: 10.1038/nmeth.f.407