TUNEL assay—Standardized method for testing sperm DNA fragmentation

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Fragmentation of sperm DNA using the TUNEL method.

OBJECTIVES To establish the validity of the TUNEL assay in determining sperm DNA fragmentation, the relationship between the degree of fragmentation and the seminal parameters and the sample needed to conduct the test. MATERIAL AND METHODS We used semen samples from healthy fertile men (n=33), patients who consulted for infertility with a prescription for the TUNEL assay (n=77) and patients w...

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tau.amegroups.com © Translational Andrology and Urology. All rights reserved. Dr. Rey, in his commentary (1), has provided us with a comprehensive summary of the practice recommendations proposed by Agarwal et al. (2) with additional discussion on the basic physiology of sperm DNA structure. The pros and cons of the eight available sperm DNA fragmentation (SDF) tests were listed by the author. ...

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ژورنال

عنوان ژورنال: Andrologia

سال: 2020

ISSN: 0303-4569,1439-0272

DOI: 10.1111/and.13738