TRYPTOPHAN SYNTHESIS FROM SERINE BY RUMEN MICROORGANISMS IN VITRO
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چکیده
منابع مشابه
Effect of Different Ratios of Concentrate and Roughage on Lipid Synthesis by Rumen Microorganisms In Vitro
The effect of different feeding ratios of concentrate to roughage on ruminal lipid synthesis in vitro was examined. Three sheep fitted with a rumen fistula were fed three different ratios (8:2, 4:6 and 0:10) of concentrate and roughage, and their rumen liquor were used for incubation. C-labeled glucose or sodium acetate as substrate was added to cultures of rumen liquor, and they were incubated...
متن کاملMetabolism of glycine by rumen microorganisms.
Rumen microorganisms rapidly metabolized glycine at rates varying from 0.014 to 0.241 mumole of glycine per ml per min. The main metabolic products were carbon dioxide, acetic acid, and ammonia; little glycine was incorporated into bacterial protein. Use of carboxyl or methylene-labeled glycine showed that the carbon dioxide came mainly from the carboxyl of glycine, whereas both carbons of acet...
متن کاملDissimilation of tryptophan and related indolic compounds by ruminal microorganisms in vitro.
Intraruminal doses of L-tryptophan cause acute pulmonary edema and emphysema in cattle. The D and L isomers of tryptophan and 22 related indolic compounds were incubated with ruminal microorganisms in vitro. Incubation of L-[U-benzene ring-(14)C]tryptophan with ruminal microorganisms for 24 h resulted in 39% of the added radioactivity being incorporated into skatole, 7% into indole, and 4% into...
متن کاملTryptophanase-Catalyzed l-Tryptophan Synthesis from d-Serine in the Presence of Diammonium Hydrogen Phosphate
Tryptophanase, an enzyme with extreme absolute stereospecificity for optically active stereoisomers, catalyzes the synthesis of l-tryptophan from l-serine and indole through a beta-substitution mechanism of the ping-pong type, and has no activity on d-serine. We previously reported that tryptophanase changed its stereospecificity to degrade d-tryptophan in highly concentrated diammonium hydroge...
متن کاملpreliminary study on cost-effective l-tryptophan production from indole and l-serine by e. coli cells
background: l-tryptophan is used widespread in the pharmaceutical industry. the majority of l-trp production depends on microbial processes that produce l-tryptophan from indole and l-serine. these processes are very costly due to the costs of precursors, especially l-serine. use of inexpensive substitutions as the l-serine source of l-tryptophan production enables us to reach a cost-effective ...
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ژورنال
عنوان ژورنال: Canadian Journal of Animal Science
سال: 1972
ISSN: 0008-3984,1918-1825
DOI: 10.4141/cjas72-081