منابع مشابه
Troponin I, the story.
Featured Article: Bodor GS, Porter S, Landt Y, Ladenson JH. Development of monoclonal antibodies for an assay of cardiac troponin I and preliminary results in suspected cases of myocardial infarction. Clin Chem 1992;38:2203–14. In the early 1980s, David N. Dietzer and I started building a team to develop monoclonal antibodies to the isoenzymes then used for detecting cardiac damage: creatine ki...
متن کاملThe solution structure of a cardiac troponin C-troponin I-troponin T complex shows a somewhat compact troponin C interacting with an extended troponin I-troponin T component.
We have investigated the structure of the cTnC-cTnI-cTnT(198-298) calcium-saturated, ternary cardiac troponin complex by small-angle scattering with contrast variation. Shape restoration was also applied to the scattering information resulting from the deuterated cTnC subunit, the unlabeled cTnI-cTnT(198-298) subunits, and the entire complex. The experimental results and modeling indicate that ...
متن کاملChimeric cardiac troponin I antibodies
most assay manufacturers take additional measures during the assay development. The most frequent procedure involves suppressing the non-specific binding with blocking reagents, which vary from normal mouse IgG to more refined formulations. Whilst in most cases these measures are adequate, they do result in additional costs in terms of both material and labor. The effect of HAMA can also be sup...
متن کاملHydrodynamic properties of bovine cardiac troponin-I and troponin-T.
Bovine cardiac troponin-I (TN-I) and troponin-T (TN-T) have been examined in solution using ultracentrifugation, gel filtration, and viscosity. A new method of purifying TN-T, employing hydroxylapatite chromatography in 6 M urea, is reported. Cardiac TN-T (Mr = 36,000) undergoes a reversible, concentration-dependent association in nondenaturing buffers, probably to a tetramer. The Stokes radius...
متن کاملWhich Troponin I Peak Concentration?
To the Editor:—We read with interest the article of Conzen et al. demonstrating a reduction of cardiac troponin I release after coronary artery bypass grafting on the beating heart when sevoflurane was compared to propofol. Most previous studies identified peak postoperative troponin I as the major endpoint. It is not clear from the article of Conzen et al. which was the peak postoperative trop...
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ژورنال
عنوان ژورنال: Circulation Research
سال: 2002
ISSN: 0009-7330,1524-4571
DOI: 10.1161/01.res.0000035340.44036.a0