Triclinic crystals associated with fibers of deoxygenated sickle hemoglobin.

Deoxygenated sickle hemoglobin. Effects of lyotropic salts on its solubility.

The effects of 22 lyotropic salts on the polymerization of deoxygenated sickle hemoglobin (deoxy-Hb S) were evaluated. The equilibrium solubility was measured as the saturation concentration, cBat, after phase separation by centrifugation at 30°C. The linear plots of csat uersus salt concentration allowed the assignment of particular cations or anions as either salting-in (csat increased) or sa...

Intraerythrocytic Hemoglobin Crystals in Sickle Cell-hemoglobin C Disease.

Permeability characteristics of deoxygenated sickle cells.

This study investigated the effect of acute deoxygenation on membrane permeability characteristics of sickle cells. Measured fluxes of Na+ and K+ in ouabain-inhibited cells, of chloride and sulfate exchange in 4,4'-diisothiocyanostilbene-2,2'-disulfonate (DIDS)-inhibited and untreated cells, and of erythritol, mannitol, and arabinose in cytochalasin B-inhibited cells indicated that a deoxygenat...

Structure of sickled erythrocytes and of sickle-cell hemoglobin fibers.

Deoxyhemoglobin from patients homozygous for sickle-cell anemia (deoxyhb S) aggregates into long straight fibers. These may extend through most of the length of the sickled cell, forming either square or hexagonally packed bundles with lattice constants of 170-180 A. Each fiber is a tube made up of six thin filaments, which are wound around the tubular surface with a helical pitch of about 3000...

Ca2+ permeability in deoxygenated sickle cells.

Deoxygenation of sickle cells is known to increase cation permeabilities (Na+, K+, and Ca2+). The possible mechanisms involved in the increased uptake of Ca2+ were investigated: activation of Ca2+ channels, involvement of the anion channel, and the formation of endocytic vacuoles. The Ca2+ channel blocker nifedipine reduced the deoxy-stimulated Ca2+ uptake by about 30% to 40%. The anion channel...