Transmembrane routes of cationic liposome-mediated gene delivery using human throat epidermis cancer cells

Cationic liposome-mediated gene delivery in vivo.

Several improvements have been made in liposomal delivery, thus making this technology potentially useful for treatment of certain diseases in the clinic. Success in non-viral delivery is complicated and requires optimization of several components. These components include nucleic acid purification, plasmid design, formulation of the delivery vehicle, administration route and schedule, dosing, ...

Cationic liposome-mediated CXCR4 gene delivery into hematopoietic stem/progenitor cells: implications for clinical transplantation and gene therapy.

The chemokine stromal cell-derived factor (SDF)-1α/CXCL12 and its receptor CXC chemokine receptor 4 (CXCR4) play a crucial role in the homing/engraftment and retention of hematopoietic stem/progenitor cells (HSPCs) in the bone marrow. It has been shown using the viral gene transfer technique that CXCR4 overexpression on human CD34(+) HSPC significantly improves their engraftment in murine model...

Cationic liposome-mediated RNA transfection.

We have developed an efficient and reproducible method for RNA transfection, using a synthetic cationic lipid, N-[1-(2,3-dioleyloxy)propyl]-N,N,N-trimethylammonium chloride (DOTMA), incorporated into a liposome (lipofectin). Transfection of 10 ng to 5 micrograms of Photinus pyralis luciferase mRNA synthesized in vitro into NIH 3T3 mouse cells yields a linear response of luciferase activity. The...

Advances in cationic lipid-mediated gene delivery

Benjamin Martin, Abderrahim Aissaoui, Matthieu Sainlos, Noufissa Oudrhiri, Michelle Hauchecorne, Jean-Pierre Vigneron, Jean-Marie Lehn and Pierre Lehn 1 Laboratoire de Chimie des Interactions Moléculaires, CNRS UPR 285, Collège-de-France, 11 Place Marcelin Berthelot, 75005 Paris, France. 2 INSERM U458, Hôpital Robert Debré, 48 Boulevard Sérurier, 75019 Paris, France ____________________________...

preparation and in vitro transfection of modified cationic liposome-linear polyethyleneimine-dna nanocomplexes for gene delivery