Transmembrane Insertion of the Toxoplasma gondii GRA5 Protein Occurs after Soluble Secretion into the Host Cell
نویسندگان
چکیده
منابع مشابه
Cloning, expression and immunoreactivity of recombinant Toxoplasma gondii GRA5 protein
BACKGROUND AND OBJECTIVES Toxoplasma gondii is an obligatory intracellular parasite which causes severe diseases in the fetus of pregnant women and immunocopmromised patients. Serological tests based on recombinant protein are one of the main diagnosis methods for the detection of specific antibodies in serum samples. Dense granule antigenic proteins derived from T. gondii (TgGRAs) are potentia...
متن کاملInvasion of Toxoplasma gondii occurs by active penetration of the host cell.
Toxoplasma gondii is an obligate intracellular parasite that infects a wide variety of vertebrate cells including macrophages. We have used a combination of video microscopy and fluorescence localization to examine the entry of Toxoplasma into macrophages and nonphagocytic host cells. Toxoplasma actively invaded host cells without inducing host cell membrane ruffling, actin microfilament reorga...
متن کاملAtomic resolution insight into host cell recognition by Toxoplasma gondii.
The obligate intracellular parasite Toxoplasma gondii, a member of the phylum Apicomplexa that includes Plasmodium spp., is one of the most widespread parasites and the causative agent of toxoplasmosis. Micronemal proteins (MICs) are released onto the parasite surface just before invasion of host cells and play important roles in host cell recognition, attachment and penetration. Here, we repor...
متن کاملcloning, expression and immunoreactivity of recombinant toxoplasma gondii gra5 protein
background and objectives: toxoplasma gondii is an obligatory intracellular parasite which causes severe diseases in the fetus of pregnant women and immunocopmromised patients. serological tests based on recombinant protein are one of the main diagnosis methods for the detection of specific antibodies in serum samples. dense granule antigenic proteins derived from t. gondii (tggras) are potenti...
متن کاملHost Cell Penetration of Toxoplasma gondii.
The host cell penetration of Toxoplasma gondii was studied with reference to synthesis of a penetration-enhancing factor and the penetrative capacity of the parasites during the various phases of the infectious cycle. HeLa cells as suspension or monolayer cultures were infected with Toxoplasma. After a lag phase shorter than 1 hr, there was a synthesis of penetration-enhancing factor demonstrab...
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ژورنال
عنوان ژورنال: Molecular Biology of the Cell
سال: 1999
ISSN: 1059-1524,1939-4586
DOI: 10.1091/mbc.10.4.1277