Transgenic East African Highland Banana Plants Are Protected against Radopholus similis through Host-Delivered RNAi
نویسندگان
چکیده
The burrowing nematode Radopholus similis is considered a major problem of intensive banana cultivation. It can cause extensive root damage resulting in the toppling disease banana, which means that plants fall to ground. Soaking R. double-stranded (ds) RNA genes Rps13, chitin synthase (Chs-2), Unc-87, Pat-10 or beta-1,4-endoglucanase (Eng1a) suppressed reproduction on carrot discs, from 2.8-fold (Chs-2) 7-fold (Rps13). East African Highland Banana cultivar Nakitembe was then transformed with constructs for expression dsRNA against same genes, and each construct, 30 independent transformants were tested infection. Four months after transfer vitro culture greenhouse, transferred screenhouse inoculated 2000 nematodes per plant, thirteen weeks later, they analyzed several parameters including plant growth, necrosis final population. Plants average showing lower multiplication than nontransformed controls expressing nonendogenous gene. In conclusion, RNAi seems efficiently protect caused by similis, opening perspectives control this pest.
منابع مشابه
Phenalenone-type phytoalexins mediate resistance of banana plants (Musa spp.) to the burrowing nematode Radopholus similis.
The global yield of bananas-one of the most important food crops-is severely hampered by parasites, such as nematodes, which cause yield losses up to 75%. Plant-nematode interactions of two banana cultivars differing in susceptibility to Radopholus similis were investigated by combining the conventional and spatially resolved analytical techniques (1)H NMR spectroscopy, matrix-free UV-laser des...
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I ZHAR Y., M. W YSOKI , AND L. G UR . 1979. The effectiveness of Bacillus thuringiensis Berliner on Boarmia (Ascotis) selenaria Schiff. (Lepidoptera. Geometridae) in laboratory tests and field trials. Phytoparasitica 7: 65-77. K OLLER , O. C. 1982. Abacaticultura. Editora da Universidade, Porto Alegre. 138 pp. M EDINA , J. C. 1978. Abacate: da cultura ao processamento e comercialização. Ital, C...
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ژورنال
عنوان ژورنال: International Journal of Molecular Sciences
سال: 2023
ISSN: ['1661-6596', '1422-0067']
DOI: https://doi.org/10.3390/ijms241512126