TRANSFORMATION OF PSEUDOMONAS AERUGINOSA BY PLASMID DNA
نویسندگان
چکیده
منابع مشابه
Transformation of vegetative cells of Bacillus thuringiensis by plasmid DNA.
Plasmid DNA-mediated transformation of vegetative cells of Bacillus thuringiensis was studied with the following two plasmids: pBC16 coding for tetracycline resistance and pC194 expressing chloramphenicol resistance. A key step was the induction of competence by treatment of the bacteria with 50 mM Tris hydrochloride buffer (pH 8.9) containing 30% sucrose. Transformation frequency was strongly ...
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A broad-host-range cloning vector, pUI81, was constructed in vitro from plasmids RSF1010 and pSL25 (a pBR322 derivative) and used to assay for transformation in Rhodopseudomonas sphaeroides. Washing cells with 500 mM Tris was an effective means of inducing competence for DNA uptake. Transformation frequencies as high as 10(-5) (transformants per viable cell) have been achieved by incubating Tri...
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ژورنال
عنوان ژورنال: The Journal of General and Applied Microbiology
سال: 1977
ISSN: 1349-8037,0022-1260
DOI: 10.2323/jgam.23.183