Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility

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Transformation of Monascus purpureus to hygromycin B resistance with cosmid pMOcosX reduces fertility

∗ Corresponding author Albino strain KB20M1 of Monascus purpureus was genetically transformed to hygromycin B resistance with cosmid pMOcosX, using biolistic bombardment. Conidia and mycelial fragments were used as the recipient material and rupture disk strengths of 6.2 and 9.3 MPa each yielded a transformed isolate from five bombardments. Southern analysis suggested a single copy of the cosmi...

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ژورنال

عنوان ژورنال: Electronic Journal of Biotechnology

سال: 2003

ISSN: 0717-3458,0717-3458

DOI: 10.2225/vol6-issue2-fulltext-3