Transformation of Lactobacillus helveticus subsp. jugurti with plasmid pLHR by electroporation.
نویسندگان
چکیده
منابع مشابه
rorn Lactobacillus helveticus
2 Department of Food Science, University of Wisconsi nMad ison, WI 53706, USA A prolinase (pepR) gene was cloned from an industrial Lactobacillus helveticus strain (53/7). Three clones, hybridizing with a gene probe specific for a peptidase shown to have activity against diand tripeptides, were detected from a L. helveticus genomic library constructed in Escherichia coli. None of the three clon...
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BACKGROUND Lactic acid bacteria (LAB) play an important role in agricultural as well as industrial biotechnology. Development of improved LAB strains using e.g. library approaches is often limited by low transformation efficiencies wherefore one reason could be differences in the DNA methylation patterns between the Escherichia coli intermediate host for plasmid amplification and the final LAB ...
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ژورنال
عنوان ژورنال: Agricultural and Biological Chemistry
سال: 1990
ISSN: 0002-1369,1881-1280
DOI: 10.1271/bbb1961.54.1537