Transformation of Aspergillus parasiticus using autonomously replicating plasmids from Aspergillus nidulans
نویسندگان
چکیده
منابع مشابه
Transformation of Aspergillus nidulans
Aspergillus nidulans strain G191 was transformed to hygromycin resistance using plasmid pDH25, which contains the bacterial hygromycin B phosphotransferase gene (hph) fused to promoter elements of the A. nidulans trpC gene. Southern hybridizations of transformants revealed multiple, integrated copies of the vector. A pleiotropic effect conferring increased hygromycin B sensitivity was found to ...
متن کاملTransformation of Aspergillus nidulans by using a trpC plasmid.
We constructed a chimeric plasmid carrying a complete copy of the trifunctional trpC gene from the Ascomycete fungus Aspergillus nidulans. This plasmid, designated pHY201, replicates in Escherichia coli, where it confers resistance to ampicillin and chloramphenicol and complements trpC mutants lacking phosphoribosylanthranilate isomerase activity. We used pHY201 to transform an A. nidulans trpC...
متن کاملAspergillus nidulans
Light-dependent conidiation in the filamentous ascomycete, Aspergillus nidulans, is contingent on the allelic state of the velvet (veA) gene. Light dependence is abolished by a mutation in this gene (veAl) , which allows conidiation to occur in the absence of light. We have isolated and characterized six extragenic suppressors of veAl that restore the light-dependent conidiation phenotype. Alle...
متن کاملTransformation of Aspergillus aculeatus using the drug resistance gene of Aspergillus oryzae and the pyrG gene of Aspergillus nidulans.
Transformation systems for Aspergillus aculeatus has been developed, based on the use of the pyrithiamine resistance gene of Aspergillus oryzae and the orotidine-5'-monophosphate decarboxylase gene (pyrG) of Aspergillus nidulans. An A. aculeatus mutant which can be transformed effectively by the A. nidulans pyrG gene was isolated as a transformation host. This is the first report of transformat...
متن کاملParasitical: A New Metabolite from Aspergillus parasiticus
Parasiticol, a metabolite structurally related to the aflatoxins, has been isolated and characterized. The compound is elaborated by several strains of the Aspergillus jiaDus series previously shown to be aflatoxin producers. The metabolite was separated and purified by chromatography Oll three columns (silicic acid, silica gel, and alumiua) followed by recrystallization from chlorofom,-bexane....
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ژورنال
عنوان ژورنال: FEMS Microbiology Letters
سال: 1994
ISSN: 0378-1097
DOI: 10.1016/0378-1097(94)90329-8