Transfer of Plasmids among Bacilli

Mutation among Hog-cholera Bacilli

1. In the hog-cholera group the mutation from smooth (A) to granular (B) type occurs and a mutant with a moist, opaque colony formation was noted. 2. The B mutant when obtained pure remains a B type without reversion in the stock cultures. 3. The B mutant can be made to assume some of the A characters by rapid successive transplanting in bouillon. 4. The B mutant can usually be found in older t...

Distribution of shufflon among IncI plasmids.

A shufflon or clustered inversion is a novel type of DNA rearrangement originally discovered in the IncI1 plasmid R64 (T. Komano, A. Kubo, and T. Nisioka, Nucleic Acids Res. 15:1165-1172, 1987). In a 1.95-kilobase region of R64 DNA, four DNA segments inverted independently or in groups, resulting in a complex DNA rearrangement. We found similar types of shufflon in other IncI1 plasmids, includi...

Antimicrobial Resistance among Gram-negative Bacilli

Transfer of Plasmids between Bacillus subtilis and Streptococcus lactis.

The shuttle plasmid pGK12, as well as several Staphylococcus aureus plasmids, was introduced into Streptococcus lactis by intergeneric protoplast fusion with Bacillus subtilis. The S. aureus plasmids were stably inherited in S. lactis, and so they may possibly be used as cloning vectors in lactic streptococci.

Transfer of IncP Plasmids to Extremely Acidophilic Thiobacillus thiooxidans.

The broad-host-range IncP plasmids RP4, R68.45, RP1::Tn501, and and pUB307 were transferred directly to extremely acidophilic Thiobacillus thiooxidans from Escherichia coli by conjugation at frequencies of 10 to 10 per recipient. The ability of T. thiooxidans to receive and express the antibiotic resistance markers was examined. The plasmid RP4 was transferred back to E. coli from T. thiooxidan...