Transfection of DHFR−and DHFR+mammalian cells using methotrexate-resistant mutants of mouse dihydrofolate reductase
نویسندگان
چکیده
منابع مشابه
Effects of 5-fluorouracil on dihydrofolate reductase and dihydrofolate reductase mRNA from methotrexate-resistant KB cells.
Growth of methotrexate-resistant dihydrofolate reductase gene-amplified KB cells in the presence of 5-fluorouracil results in an increase in dihydrofolate reductase mRNA. This increase can be solely attributed to a species of RNA of approximately 3.5 kilobase pairs in size. Although dihydrofolate reductase enzyme activity increases per cell with increasing 5-fluorouracil, there is a decrease of...
متن کاملDihydrofolate reductase amplification and sensitization to methotrexate of methotrexate-resistant colon cancer cells.
Gene amplification is one of the most frequent manifestations of genomic instability in human tumors and plays an important role in tumor progression and acquisition of drug resistance. To better understand the factors involved in acquired resistance to cytotoxic drugs via gene amplification, we have analyzed the structure and dynamics of dihydrofolate reductase (DHFR) gene amplification in HT2...
متن کاملSelective multiplication of dihydrofolate reductase genes in methotrexate-resistant variants of cultured murine cells. 1978.
The rate of dihydrofolate reductase synthesis in the AT3000 line of methotrexate-resistant murine Sarcoma 180 cells is approximately 200to 250-fold greater than that of the sensitive, parental line. We have purified cDNA sequences complementary to dihydrofolate reductase mRNA and subsequently used this probe to quantitate dihydrofolate reductase mRNA and gene copies in each of these lines. Anal...
متن کاملSelective Multiplication of Dihydrofolate Reductase Genes in Methotrexate-resistant Variants of Cultured Murine Cells*
The rate of dihydrofolate reductase synthesis in the AT3000 line of methotrexate-resistant murine Sarcoma 180 cells is approximately 200to 250-fold greater than that of the sensitive, parental line. We have purified cDNA sequences complementary to dihydrofolate reductase mRNA and subsequently used this probe to quantitate dihydrofolate reductase mRNA and gene copies in each of these lines. Anal...
متن کاملIsolation and characterization of the multiple forms of dihydrofolate reductase from methotrexate-resistant hamster cells.
Dihydrofolate reductase, isolated from baby hamster kidney cells by affinity chromatography, gave multiple bands on analytical gel electrophoresis and electrofocusing. The number of bands could be reduced to two by forming a complex with the enzyme inhibitor, methotrexate. The two basic enzyme forms, called Form I and Form II, were obtained in large quantities by preparative disc electrophoresi...
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ژورنال
عنوان ژورنال: FEBS Letters
سال: 1990
ISSN: 0014-5793
DOI: 10.1016/0014-5793(90)81213-8