Transfection of cells using flow-through electroporation based on constant voltage
نویسندگان
چکیده
منابع مشابه
Flow-through electroporation based on constant voltage for large-volume transfection of cells.
Genetic modification of cells is a critical step involved in many cell therapy and gene therapy protocols. In these applications, cell samples of large volume (10(8)-10(9)cells) are often processed for transfection. This poses new challenges for current transfection methods and practices. Here we present a novel flow-through electroporation method for delivery of genes into cells at high flow r...
متن کاملFlow-through Electroporation for Transfection Based on Low-frequency Ac Voltage
Department of Chemical Engineering, Virginia Tech, USA Electroporation has been demonstrated to be an efficient technique for gene transfection on mammalian cells. However, traditional technique employing direct current (DC) electrical pulses has the problem of too much electrolysis and low cell viability, which limits the application in gene delivery study. We demonstrate here flow-through ele...
متن کاملconstructing gender identity through narratives based on hallidays metafunctions
هویت, شکل دادن و بازنمایی آن در گفتمان, توجه بسیاری از محققان این رشته را به خود جلب کرده است. تحقیق حاضر بر شکل دادن به هویت جنسیتی هشت تن از دانشجویان ایرانی مشغول به تحصیل در دوره کارشناسی ارشد از طریق بررسی روایات آنان از تجربیات شخصی, متمرکز شده است. تحلیل داده ها در این تحقیق مشتمل بر سه بخش است: بخش اول شامل کدگذاری موضوعی روایات است که بر اساس آن هویت جنسیتی شرکت کنندگان در تحقیق بر اسا...
15 صفحه اولElectroporation for Transfection and Differentiation of Dental Pulp Stem Cells
Target gene delivery is needed to induce cellular differentiation or a specific therapeutic effect. Electroporation is a relatively safe and simple technique to deliver nucleic acids to the cell that acts by rendering cells transiently permeable using short periods of high voltage. In stem cell research, human dental pulp stem cells (hDPSCS) are highly accessible, and they exhibit broad differe...
متن کاملImproved transfection efficiency of 293 cells by radio frequency electroporation.
Electroporation, a widely used technique allowing the transfer of biological molecules into a variety of cell types (13), has been used with success for introducing plasmids and short oligonucleotides into mammalian, yeast and a variety of other cell types (1,2,9,10). A modification of the square-wave pulse technique that uses radio frequency (RF) electrical bursts is now being used effectively...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Nature Protocols
سال: 2011
ISSN: 1754-2189,1750-2799
DOI: 10.1038/nprot.2011.360