Transcriptomic Analysis of Medicago truncatula under Long-Day Conditions

The objective of this research was to understand the expression characteristics and biological functions Medicago truncatula genes under long-day conditions. leaves “R108” tribulus at branch stage (A), bud (B), initial flowering (C), full (D) were sequenced using RNA-Seq technology. genome truncatula, a related species used as reference for sequence comparison. transcriptomes three adjacent periods (A vs. B, B C, C D) analyzed differential gene these screened. A total 6875 differentially expressed detected. GO functional analysis showed that mainly involved in processes, cell components, molecular functions, among which most synthesis components. KEGG enrichment circadian rhythm, photosynthetic antenna protein, ribosome metabolism, other pathways. number single nucleotide variants detected by cSNP 312,875, frequency A/G C/T highest. function eggNOG divided into 23 categories, with 26,745 having similarities, while 9008 classified an unknown function, 2669 part signal transduction mechanisms, 2194 being transcription. In different developmental stages D), 3463 up-regulated 3412 down-regulated found. difference between more noteworthy stage. addition, 79 found, 51 identified participating photoperiodic regulation pathway, consisting up-regulated, 28 down-regulated. ratios gene-LOC11410562(GI), gene-LOC11435974(CO), gene-LOC11422615(TOC1), gene-LOC11432385(LHY) higher than those gene-LOC25500742(PHYA) gene-LOC11 431402(ELF3); gene-LOC11434778(Col13), gene-LOC25498015(Col6), gene-LOC11415514(Col9) pre-expressed. above significantly lays foundation further study mechanism truncatula.