Transcriptome and Metabolome Analyses of Salt Stress Response in Cotton (Gossypium hirsutum) Seed Pretreated with NaCl

Abiotic stresses adversely influence crop productivity and salt stress is one limiting factor. Plants need to evolve their defense mechanisms survive in such fluctuating scenarios at either the biochemical, physiological, or molecular level. The analytical/critical investigations of cotton (Gossypium hirsutum) plants that involve looking into transcriptomic metabolomic profiles could give a comprehensive picture response plant stress. This study was conducted on pre-treated seeds by soaking them 3% sodium chloride (NaCl) solution room temperature for 0.5, 1, 1.5 h. In total, 3738 285 differentially expressed genes (DEGs) metabolites, respectively, were discovered. prominent DEGs included AtCCC1, EP1, NHE, AtpOMT, GAST1, CLC-c, ARP, AtKIN14, AtC3H2, COP9, AtHK-2, EID1 code regulation seed growth, abscisic acid receptor PYR/PYL, cellular regarding tolerance (especially salt) germination, jasmonic acid, salicylic auxin-activated signaling pathways. A more significant amount transcription factors, including ethylene-responsive TFs ERF (205), bHLH (252), ZF-domains (167), (101), MYB (92), NAC (83), GATA (43), auxin-responsive proteins (30), MADs-box (23), bZIP (27), HHO (13) discovered samples NaCl-pretreated seedlings under different treatments. functional annotations exposed important roles regulating phytohormones signal-transduction-mediated pathways salt-treated seeds. metabolites analysis revealed differential accumulation flavonols, phenolic amino acids, derivatives seedling treated 0.5 h with NaCl. conjoint showed most associated production glucose-1-phosphate, uridine 5?-diphospho-D-glucose, 2-deoxyribose 1-phosphate conditions. These results indicated positive effects NaCl treatments seedlings’ germination seemingly activating specific growth-promoting enzymes alleviate adverse Hence, pre-treatment can be beneficial future management breeding programs enhance growth development