Transcriptome Analysis and Metabolic Profiling of Lycoris Radiata
نویسندگان
چکیده
منابع مشابه
Two new amaryllidaceae alkaloids from the bulbs of Lycoris radiata.
Two new Amaryllidaceae alkaloids, named lycoranines A (1) and B (2), were isolated from the bulbs of Lycoris radiata. Their structures were elucidated on the basis of extensive spectroscopic analysis. Compound 2 was a new-type alkaloid, which provided a new insight into the biosynthesis of alkaloids in Amaryllidaceae plants.
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Lycoris radiata is an important medicinal and ornamental plant of China. In the present study, somatic chromosome counts and karyotype analyses, which are important aspects of plant phylogeny and evolution, were performed in 466 individuals from 25 L. radiata populations by root tip squash method. Chromosome counts revealed that 10 populations were diploid (2n = 2x = 22) and 15 were triploid (2...
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A full-length cDNA (LC3'H) was obtained from a cDNA library of Lycoris radiata by DOP-PCR (degenerate oligonucleotide primer PCR), 3'race and 5'race methods. Compared with the other reported enzymes from different plants, the deduced amino acid sequence of LC3'H exhibits significant homologies to 3'-hydroxylases that are involved in the caffeic acid biosynthesis. These findings suggest that the...
متن کاملPolymorphic microsatellite loci for the genetic analysis of Lycoris radiata (Amaryllidaceae) and cross-amplification in other congeneric species.
Lycoris radiata is a perennial herb that has been used in traditional Chinese medicine for a long time and has two main medicinal components in its bulb, lycorine and galanthamine. However, the original microsatellite loci have not been developed for any species of Lycoris. Total genomic DNA was extracted from fresh bulbs using a modified CTAB protocol. We isolated 10 microsatellite loci ...
متن کاملCytotoxic and antimalarial amaryllidaceae alkaloids from the bulbs of Lycoris radiata.
Phytochemical investigation of the 80% ethanol extract of the bulbs of Lycoris radiata resulted in the isolation of five new Amaryllidaceae alkaloids: (+)-5,6-dehydrolycorine (1), (+)-3α,6β-diacetyl-bulbispermine (2), (+)-3α-hydroxy-6β-acetyl- bulbispermine (3), (+)-8,9-methylenedioxylhomolycorine-N-oxide (5), and 5,6-dihydro-5- methyl-2-hydroxyphenanthridine (7), together with two known compo...
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ژورنال
عنوان ژورنال: Biology
سال: 2019
ISSN: 2079-7737
DOI: 10.3390/biology8030063