Transcriptional responses of

Hypericum perforatum L. (St. John’s wort) is a well-known medicinal plant that possesses secondary metabolites with beneficial pharmacological properties. However, improvement in the production of via genetic manipulation challenging task as H. remains recalcitrant to Agrobacterium tumefaciens-mediated transformation. Here, transcripts key genes involved several defence responses (secondary metabolites, RNA silencing, reactive oxygen species (ROS) and specific genes) were investigated suspension cells inoculated A. tumefaciens by quantitative real-time PCR. Results indicated from xanthone, hypericin melatonin biosynthesis pathways, ROS-detoxification enzyme HpAOX, well Hyp-1 HpPGIP, all upregulated rapidly respond elicitation perforatum. By contrast, expression levels hyperforin flavonoid pathways markedly downregulated upon elicitation. In addition, we compared patterns leaf tissues without dark glands, major site metabolite production. Overall, provide evidence for upregulation phenylpropanoid pathway response Agrobacterium, suggesting could modulate recalcitrance