Transcriptional analysis reveals formation of axillary solitary hook in vine plant Uncaria rhynchophylla

Abstract The hook of Uncaria rhynchophylla is an important external phenotype representing the quality Gou-Teng. formation pattern U. displays a unique feature that solitary and opposite hooks alternately occur in leaf axils forming liner series. only grows on lower side axil, resulting from decline total yield hooks. However, reasons for are not clear. Therefore, survey molecular mechanisms needs to be performed urgently. We obtained 250.67 million high-quality clean reads axillary stem segments (HS) upper (HSCK) libraries. detected 42 DEGs (differentially expressed genes) between HS HSCK libraries; 11 were down-regulated, 31 up-regulated. Gene ontology functional classification indicated they included two genes encoded LAZY1 related regulation polar auxin transport, AUX/IAA AP2/ERF plant hormone signaling transduction, F3H flavone synthase activity. These six associated with lateral transport (PAT) regulation, their expression level had high correlation IAA distribution upon gravity stimulation. And inhibition development was observed after treatment N -1-naphthylphthalamic acid (NPA) or quercetin, which known as inhibitor. above findings might provide new information about PAT bud process under gravity.