Transcription elongation: The ‘Foggy’ is lifting…

Transcription elongation: The ‘Foggy’ is lifting…

Eukaryotic messenger RNAs are synthesized by the multisubunit enzyme RNA polymerase II, aided by myriad cofactors that control different events in this multistep process. A complete round of transcription involves: the recruitment of polymerase and general transcription factors to the promoter, RNA chain initiation and polymerase escape from the promoter, RNA chain elongation, and finally termi...

Transcription factor E is a part of transcription elongation complexes.

A homologue of the N-terminal domain of the alpha subunit of the general eukaryotic transcription factor TFE is encoded in the genomes of all sequenced archaea, but the position of archaeal TFE in transcription complexes has not yet been defined. We show here that TFE binds nonspecifically to single-stranded DNA, and photochemical cross-linking revealed TFE binding to a preformed open transcrip...

Transcription elongation

SnapShot: Transcription Elongation

Transcription elongation is a key regulatory step in gene expression that is controlled by many proteins, and mechanisms, ranging from RNA Polymerase II pausing to cotranscriptional histone modifications.

Single molecule transcription elongation.

Single molecule optical trapping assays have now been applied to a great number of macromolecular systems including DNA, RNA, cargo motors, restriction enzymes, DNA helicases, chromosome remodelers, DNA polymerases and both viral and bacterial RNA polymerases. The advantages of the technique are the ability to observe dynamic, unsynchronized molecular processes, to determine the distributions o...