Transbilayer lipid distribution in nano scale
نویسندگان
چکیده
منابع مشابه
Transbilayer distribution of lipids at nano scale.
There is a limited number of methods to examine transbilayer lipid distribution in biomembranes. We employed freeze-fracture replica-labelling immunoelectron microscopy in combination with lipid-binding proteins and a peptide to examine both transbilayer distribution and lateral distribution of various phospholipids in mammalian cells. Our results indicate that phospholipids are exclusively dis...
متن کاملDynamic transbilayer lipid asymmetry.
Cells have thousands of different lipids. In the plasma membrane, and in membranes of the late secretory and endocytotic pathways, these lipids are not evenly distributed over the two leaflets of the lipid bilayer. The basis for this transmembrane lipid asymmetry lies in the fact that glycerolipids are primarily synthesized on the cytosolic and sphingolipids on the noncytosolic surface of cellu...
متن کاملPhospholipids of lipid-containing bacteriophages and their transbilayer distribution
............................................................................................................ 8 REVIEW OF THE LITERATURE ............................................................................ 9 1.
متن کاملTransbilayer Lipid Interactions Mediate Nanoclustering of Lipid-Anchored Proteins
Understanding how functional lipid domains in live cell membranes are generated has posed a challenge. Here, we show that transbilayer interactions are necessary for the generation of cholesterol-dependent nanoclusters of GPI-anchored proteins mediated by membrane-adjacent dynamic actin filaments. We find that long saturated acyl-chains are required for forming GPI-anchor nanoclusters. Simultan...
متن کاملTransbilayer distribution of sterols in mycoplasma membranes: a review.
The polyene antibiotic, filipin, binds to 3 beta-hydroxysterols. The initial rate of filipin-sterol association, monitored in a stopped-flow spectrophotometer, was first order in each reacting partner. The ratio of rate constants in intact mycoplasma cells relative to isolated, unsealed membranes provides an estimate of sterol distribution in the membrane bilayer. Cholesterol is distributed sym...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Journal of Cell Science
سال: 2015
ISSN: 1477-9137,0021-9533
DOI: 10.1242/jcs.163105