منابع مشابه
Tracking transcripts
Next vesicle SNAP'd up ecretory vesicles wishing to play follow-the-leader use a particular SNARE to show them where the leader went, as shown by Takahashi et al. on page 255. Vesicles heading to the plasma membrane sometimes selectively bind to another that has already fused there. This process, called sequential exocytosis, is efficient at large-scale secretion because vesicles in the cytosol...
متن کاملFluorescent RNA cytochemistry: tracking gene transcripts in living cells.
The advent of jellyfish green fluorescent protein and its spectral variants, together with promising new fluorescent proteins from other classes of the Cnidarian phylum (coral and anemones), has greatly enhanced and promises to further boost the detection and localization of proteins in cell biology. It has been less widely appreciated that highly sensitive methods have also recently been devel...
متن کاملTracking germinal center B cells expressing germ-line immunoglobulin gamma1 transcripts by conditional gene targeting.
Germinal centers (GCs) represent the main sites for the generation of high-affinity, class-switched antibodies during T cell-dependent antibody responses. To study gene function specifically in GC B cells, we generated Cgamma1-cre mice in which the expression of Cre recombinase is induced by transcription of the Ig gamma1 constant region gene segment (Cgamma1). In these mice, Cre-mediated recom...
متن کاملNoncoding RNA transcripts.
Recent analyses of the human genome and available data about the other higher eukaryotic genomes have revealed that, in contrast to Eubacteria and Archaea, only a small fraction of the genetic material (ca 1.5%) codes for proteins. Most of genomic DNA and its RNA transcripts are involved in regulation of gene expression, which can be exerted at either the transcriptional level, controlling whet...
متن کاملPredicting full-length transcripts.
genes in genomic DNA. Curr. Opin. Struct. Biol. 8, 346–354 10 Stormo, G.D. (2000) Gene-finding approaches for eukaryotes. Genome Res. 10, 394–397 11 Reese, M.G. et al. (2000) Genome annotation assessment in Drosophila melanogaster. Genome Res. 10, 483–501 12 Gelfand, M.S. et al. (1996) Gene recognition via spliced sequence alignment. Proc. Natl. Acad. Sci. U. S. A. 93, 9061–9066 13 Florea, L. e...
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ژورنال
عنوان ژورنال: Journal of Cell Biology
سال: 2004
ISSN: 1540-8140,0021-9525
DOI: 10.1083/jcb1652iti5